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Full sequence genetic testing

Ziggurat

Penultimate Amazing
Ziggurat
Joined
Jun 19, 2003
Messages
61,584
I'm considering buying a full sequence genetic test for myself. A lot of the more well-known genetic testing sites like 23andme don't do full sequencing, they only target specific genes for testing. I'd like to get the whole sequence done, so I can check for rare genetic conditions and also have the data for the future. There's a few companies out there which offer this service, such as sequencing.com or nebula.org, but I'm not really familiar with any of them.

Does anyone here have experience getting a whole sequence DNA test? If so, who did you get it from, and what was your experience like? Any specific recommendations, either to go with or to avoid?
 
Family Tree DNA. It's quite expensive but they are currently running a 'special offer'. Very different from 23andme, more research oriented. Once you get the raw data you can upload it to GEDcom who do all of the specific gene testing stuff. GEDcom are known to help crime agencies so care needed if you are into ethics.
 
Vixen said:
Family Tree DNA. It's quite expensive but they are currently running a 'special offer'.
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I don't think that's full sequence DNA testing, because they don't advertise that it's full sequence. And it's not expensive, it's cheap compared to the companies I've seen advertising that they do full sequence.
 
Ziggurat said:
I don't think that's full sequence DNA testing, because they don't advertise that it's full sequence. And it's not expensive, it's cheap compared to the companies I've seen advertising that they do full sequence.
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They do a full sequence mtDNA (mitochrondial) which I think may only be available for females.
 
The Great Zaganza said:
What exactly would be the point?
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There's a few specific genes I'm interested in (for example, I have a family history of celiac but don't currently have it myself, but I'd like to know if I've got the genes). But I'm also just curious about what it can show, what's lurking in there that I haven't even thought of. And I figure if I'm going to get the testing done, might as well not half-ass it. Get all the genes sequenced, and as we learn more about the effects of different genes, I can go back and look at it without having to get retested.
 
Vixen said:
They do a full sequence mtDNA (mitochrondial) which I think may only be available for females.
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That doesn't make sense. Mitochondial DNA is only passed on through the maternal line so that will only tell you about your maternal ancestry, but everyone's got mitochondrial DNA. A cheek swab or a blood draw is going to provide just as much mtDNA for males as females, the only difference is in the gametes.
 
The Great Zaganza said:
What exactly would be the point?
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It is useful for those interested in inherited health conditions, (I was informed I may have some kind of Vitamin B deficiency which could be recitfied by a supplement) also, the more scientific side of things rather than nationality. For example, I was able to get an analysis of ancient DNA via GEDcom by uploading the raw data from FamilyTreeDNA.

BY AGE Specimen Years Old % Composition
F999902 Altai Neanderthal 50,000 0.66%
F999935 Ust-Ishim, Siberia 45,000 16.69%
Vi33 26 Neanderthal, Vindija cave, Croatia 38,100 0.44%
Vi33 16 Neanderthal, Vindija cave, Croatia 38,100 0.21%
Vi22 25 Neanderthal, Vindija cave, Croatia 38,100 0.34%
F999936 Kostenki14, Russia 37,000 3.95%
F999903 Denisova, Siberia 30,000 0.68%
F999914 Mal’ta Siberia 24,000 1.39%
F999919 Clovis Anzick-1, Montana 12,500 10.70%
F999918 Loschbour Lux 8,000 13.43%

BY PROPORTION Age (Years) % porportion mtDNA
1 F999933 BR2 Hungary 3,200 18.56% K1a1a
2 F999937 NE1 Hungary 7,200 17.40% U5b2c
3 F999916 LBK Stuttgart 7,000 17.23% T2c2
4 F999935 Ust-Ishim, Siberia 45,000 16.69% R
5 F999950 RISE493, Russia 3,200 13.97% C4a1c
6 F999918 Loschbour Lux 8,000 13.43% U5b1a
7 F999919 Clovis Anzick-1, Montana 12,500 10.70% D4h3a
8 F999957 RISE497, Russia 2,000 9.82% A2f2*
9 F999950 RISE505, Russia 3,300 9.68% U4a1b
10 F999941 RISE98, Sweden 3,700 8.69% K1b1a


(There are 38 lines but I have only included ten, for brevity, which I put into order myself.) The Clovis-Anzik one is the DNA of the ancient remains found recently in Montana of a young Native American boy and which means we are somehow related way back in the mists of time (the Amerindians are believed to have reached the American continent via the Bering Strait when it was still a land mass). How it matches with various archeological discoveries; ancient graves and their DNA analysed.

How it compares to the rest of the world (table too large).

So, the interest is in, health and medical, history and geography that goes back way beyond a couple of hundred years, for example Neolithic.

There was a great website forum called Anthrogenica which sadly closed down in August this year.
 
Ziggurat said:
That doesn't make sense. Mitochondial DNA is only passed on through the maternal line so that will only tell you about your maternal ancestry, but everyone's got mitochondrial DNA. A cheek swab or a blood draw is going to provide just as much mtDNA for males as females, the only difference is in the gametes.
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Well, they have a 'sale' on until 3 January.

Screenshot 2023-12-21 172040 by Username Vixen, on Flickr

Not spam - for information only.
 
Vixen said:
Well, they have a 'sale' on until 3 January.
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Not really the one I want. I'm interested in health stuff, I don't really care much about the ancestry stuff. That's why I want the full sequence.

And doing a full sequence of mitochondrial DNA is a lot easier than a full sequence of your nuclear DNA. Your mitochondria has less than 17k base pairs, but your nuclear DNA has about 3.3 billion base pairs (59 million base pairs on Y chromosome alone). That's my, even though they advertise doing full mitochondrial sequencing, they don't advertise doing full Y chromosome sequencing even though they charge three times as much for the top of the line paternal testing than the maternal testing.
 
Ziggurat said:
Not really the one I want. I'm interested in health stuff, I don't really care much about the ancestry stuff. That's why I want the full sequence.

And doing a full sequence of mitochondrial DNA is a lot easier than a full sequence of your nuclear DNA. Your mitochondria has less than 17k base pairs, but your nuclear DNA has about 3.3 billion base pairs (59 million base pairs on Y chromosome alone). That's my, even though they advertise doing full mitochondrial sequencing, they don't advertise doing full Y chromosome sequencing even though they charge three times as much for the top of the line paternal testing than the maternal testing.
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That's one to avoid, then <g>.

Let us know if the companies you mention in the OP add any significant knowledge to 23+me or FamilyTree DNA in terms of health issues and risks.
 
I wasn't considering it because I had partial sequencing don't at 23and me.

But I looked at sequencing.com and notice they currently have an 80% off offer for the holidays. Exclusive Holiday WGS Super Bundle $1995 $399.

Did you see that?
 
sir drinks-a-lot said:
I wasn't considering it because I had partial sequencing don't at 23and me.

But I looked at sequencing.com and notice they currently have an 80% off offer for the holidays. Exclusive Holiday WGS Super Bundle $1995 $399.

Did you see that?
Click to expand...

Yes, that's one of the ones I'm considering. But I was hoping someone here had some experience with one of these companies.
 
I haven't done full sequence DNA testing, but I am interesting in doing so. If you learn anything from this experience, Zig, I'd appreciate updates in the thread. :)
 
Vixen said:
They do a full sequence mtDNA (mitochrondial) which I think may only be available for females.
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This is more complex than you think.

You inherit about 200 mitochondria from your mother (the number of mitochondria in an ovum. The number of mitochondria in human cells varies from hundreds to thousands, depending on the cell type. Each mitochondria can differ in its genetics, and they do not evenly replicate, so different cells may have differing ratios of mitochondria with differing genetics.

https://isogg.org/wiki/Heteroplasmy

https://www.nature.com/articles/s41586-023-06426-5
 
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