It can be said that all four gospels are anti-Semitic to a degree. The book of Matthew (which, like all of the gospels, was almost certainly not written by the author to whom it is attributed) seems to be directed primarily to a Jewish audience with a potential aim of converting Jews to a Christ-modified version of Judaism. In accord with this aim, whoever wrote the book went overboard in trying to show that the coming of Jesus fulfilled Old Testament prophesy after Old Testament prophesy. There are many passages that are disparaging to the Jewish authorities.
Perhaps the most harmful passage in the Bible--the one that for thousands of years caused Christians to treat Jews as "Christ killers" for voluntarily accepting responsibility for the death of Jesus--is in the book of Matthew: "His blood be on us, and on our children!" (Mt. 27:25)
That said, the book of Luke, which appears to have Gentiles as its target audience, is even more disrespectful to the Jews. In Luke, Pilate protests Jesus's innocence three times (equal to the number of protests in the books of Matthew and Mark combined) and Luke is also the only gospel in which Pilate tries to pass of the responsibility for punishment to Herod Antipas (Luke 23:7).
The book of John is even more anti-Jewish than Luke, portraying the Jews in a very unfavorable manner. By the time this book had been written, Judaism and Christianity had almost certainly split apart, with some animosity on both sides. In this book, "the Jews" (not merely the Pharisees, Sadducees and scribes, but "the Jews" as a whole) get on Jesus's case, hassling him, threatening to kill him and plotting against him. The book of John blurs the distinction between the Jews as a group and the political/religious leaders.
In the book of John, Pilate is even more reluctant to condemn Jesus. It is only after "the Jews" threaten to go over Pilate's head to the Roman authorities and accuse Pilate of treason (John 19:12-15) that Pilate issues the order to crucify.